Data · dataset · 2026
<p>Biochemical effects of suppressor mutations on the biochemical activities of the ORC4R protein.</p>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1371/journal.pone.0349465.g003
<p>A) ATPase activity of WT ORC and ORC4R protein with or without the indicated <i>sofa</i> mutation.
Description
Hydrolysis was monitored using radioactive ATP. Percent hydrolysis (ADP/ (ADP + ATP), arbitrary units) was determined over a time course.
Plots display the mean and standard deviation of three replicates as points and errors bars, respectively. B) Electrophoretic-mobility shift assay to determine <i>ARS1</i> DNA binding of mutant protein complexes. Representative experiments are shown for each experimental set.
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Bound fraction was normalized within each gel to the WT + ATP reference and plotted for three replicates of each experimental set. WT data is displayed twice. C) <i>In vitro</i> helicase-loading assay for WT and ORC4R protein without or with the indicated suppressor mutation.
Potassium glutamate concentration was 350mM. Representative experiment is shown. Percent of WT was quantified for three replicates.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0349465.g003 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Biochemistry and cell biology · Biochemistry and cell biology · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Life Sciences · Life Sciences · Life Sciences · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae
Provenance · 3 source records, 20 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34035576 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34035576 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34035576 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:group:3101 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[organism].NCBITaxon:4932 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |