Identification and bioactivity evaluation of a novel peptide, QUB-2603, from the skin secretion of the black-striped frog, Hylarana nigrovittata

First, employ matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry to analyze and validate the structure of the peptide to ensure a successful synthesis. Then, utilize reversed-phase high performance liquid chromatography (RP-HPLC) to purify the final peptide product. In order to assess the bioactivity of QUB-2603, a variety of experiments were performed, including antimicrobial assays, cell viability assays, and haemolysis assays.

The findings demonstrated that QUB-2603 shown antibacterial properties against S. aureus (with a minimum inhibitory concentration of 2µM), E. coli (with a minimum inhibitory concentration of 4µM), and C. albicans (with a minimum inhibitory concentration of 4µM). Additionally, it had good anti-cancer properties(IC50=2.65 μM). However, it also demonstrated significant hemolytic capacity(HC50=23.17 μM).QUB-2603 shows promise as a potential antibacterial medication candidate for further investigation, based on these findings.<br><br><i>Thesis is embargoed until 31 December 2029.</i>