Genetically modifying the RNA-binding protein Regnase-1 reveals its critical role in Treg homeostasis and functions in vivo [HyperTRIBE]

By discovery of RNAs where adenosines have been edited to iosines, the RNAs that were bound by the protein of interest and then edited by ADAR are identified genome-wide.

We conducted this analyis for Regnase-1, an RNA binding protein with multiple described cellular functions, including mRNA degradation and T cell activation. Because Regnase-1 can degrade the mRNA quickly, we used the nuclease-dead mutant Reg1-D141N to conduct this assay in activated CD4+ T cells from wild-type mice.