<p>Comparison of CXC- Chemokine Expression in Response to IFNγ.</p>

Log fold change in gene expression of <i>CXCL9, CXCL10,</i> and <i>CXCL11</i> was calculated relative to untreated group and normalized to β-actin. <b>(A)</b> Individual donor (colored dotted lines) and average donor gene induction (solid black line) of <i>CXCL9, CXCL10,</i> and <i>CXCL11</i> in response to IFNγ by primary donor keratinocytes. <b>(B)</b> Fold change in <i>CXCL9, CXCL10,</i> and <i>CXCL11</i> expression. <b>(C and D)</b> Cells were mock-treated or treated with 1.0 or 100 U/mL IFNγ for 12 hours, then CXCL9, CXCL10, and CXCL11 protein was detected in cell culture supernatant using Luminex assay. <b>(C)</b> Bars represent average mean protein per donor cohort; symbols represent means of individual donors.

Dotted line for C and D represents a calculated limit of detection. <b>(D)</b> Each line represents an individual donor’s response to 0 U, 1 U and 100 U/mL IFNγ 12 hours post treatment <b>(A-D)</b> Blue bars represent asymptomatic cohort and magenta bars represent ulcerative cohort. ^*<i>P</i> < 0.05, ^** <i>P</i> < 0.01, ^*** <i>P</i> < 0.001, NS, not significant; linear mixed models were used to analyze fold change in gene expression.