Data · dataset · 2026
Spectrum-effect relationship between UPLC fingerprints and anti-inflammatory activity of Glycyrrhiza glabra L.
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This study aims to investigate the anti-inflammatory active components of Glycyrrhiza glabra L. (G. glabra) by integrating UPLC fingerprinting with spectrum-effect relationship analysis.
Description
UPLC fingerprints of 20 batches were established, characterizing 32 common peaks, four of which were identified by reference standard. There were variations in the NO inhibitory activity among different origins of G. glabra.
Combined with similarity evaluation, cluster analysis and principal component analysis, the samples were classified into two distinct categories. An in vitro cellular inflammation model was established using lipopolysaccharide-induced RAW 264.7 cells, and nitric oxide (NO) release was quantified by the Griess method to calculate the inhibition rate as the anti-inflammatory activity index. Grey relationship analysis, Pearson correlation analysis and Orthogonal projections to latent structures methods were used to explore the spectral relationship between the fingerprints of G. glabra and its anti-inflammatory activity.
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The results demonstrated that P5 (isoliquiritin), P8 (isoformononetin), P12 (licoricesaponin G2) and P16 (glycyrrhizic acid) were positively correlated with NO inhibitory activity. Molecular docking revealed favorable binding affinities of these compounds toward inducible nitric oxide synthase (iNOS). Subsequent cell-based validation at non-cytotoxic concentrations confirmed potent NO suppression, with IC50 values of (17.41±0.52 μmol/L), (25.22±0.84 μmol/L), (59.79±1.12 μmol/L), (39.67±1.06 μmol/L), respectively.
These findings indicate the anti-inflammatory effects of G. glabra result from synergistic multi-component action, and the identified constituents (isoliquiritin, isoformononetin, licoricesaponin G2, glycyrrhizic acid) are the key active compounds underlying its therapeutic efficacy.
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Where it is published
- DOI doi.org/10.57760/sciencedb.nprd.000db ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
Related
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.nprd.000db | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |