Imaging · dataset · 2023
P bodies in HeLa, A549 and BJ cells – single-cell feature values
Listed in Teesside University Research Data Repository
Description
Data in the paper "Cellular state landscape and herpes simplex virus type 1 infection progression are connected" by Pietilä et al., 2023: - Single-cell DDX6 mean intensity, processing body (PB) count and PB sum and mean area in HeLa and A549 cells - A549_pBodies_two_replicates.csv (Figure 7g and Supplementary 14c) - HeLa_pBodies_second_replicate.csv (Supplementary Figure 14a,b) Cells were infected with HSV-1, and after fixation markers were detected by immunofluorescence imaging.
P bodies were segmented, counted, and their mean area per cell was quantified. Cells were classified into uninfected and infected cells as described in the paper. Data cleanup and normalisation were applied as described in the paper.
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HeLa data are from one experiment. A549 data are from two independent experiments. Experimental conditions are in duplicate or triplicate (indicated by “well_name”).
Links
Where it is published
- DOI doi.org/10.17632/mg73d32md4.1 ↗
DOI / persistent id · from researchdata tees ac uk
Catalogue records · 1
- OAI-PMH record data.mendeley.com/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Adata… ↗
metadata API · from researchdata tees ac uk
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Microscopy · Social Science
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Teesside University Research Data Repository | oai:data.mendeley.com/mg73d32md4.1 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · researchdata tees ac uk | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].local:field:earth-environmental | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| concepts[modality].local:modality:imaging | enrichment · researchdata tees ac uk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:microscopy | mapping · researchdata tees ac uk | vocabulary-mapper@1.0.0 | keywords['Fluorescence Microscopy'] |
| description | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | |
| title | source · researchdata tees ac uk | connector:researchdata_tees_ac_uk@1.0.0 | /metadata/dc/title |