<p>The low temperature-induced acyltransferase ZDHHC15a mediates palmitoylation of SVCVG.</p>

Then, the precipitated proteins were analyzed by Western blotting. <b>(E-F)</b> ZF4 cells were transfected with si-ZDHHC15a or si-NC for 12 h and then infected with SVCV(MOI = 10) for 24 <b>h.</b> Cell samples and supernatant were collected 24 h after infection. The viral RNA abundance was detected using qRT-PCR. SVCV titers were measured by Viral plaque assay. <b>(G)</b> Percentage survival of wild-type and <i>zdhhc15a</i>^-/- zebrafish (n = 30) injected with 10 µl SVCV (10⁷PFU/ml).

Statistics of survival rate were calculated with the log-rank (Mantel-Cox). ***<i>p</i> < 0.001. <b>(H)</b> The virus titer in tissues were detected by virus titration in zebrafish. Data was presented as mean ± SD from three independent experiments. Statistical analysis was performed using Student’s <i>t</i>-<i>t</i>est between different groups and the asterisk (*) indicates significant differences between groups*p < 0.05, **p < 0.01, ***p < 0.001.</p>