Data · dataset · 2021
13C-Labelling data for HCT116 Glc- Gal- and SURF1 KO-cells
Listed in ScienceDB
This data set comprises the GC-MS raw data for analysis of the metabolism of HCT116 wild type cells in Glucose- and Galactose-containining medium as well as the SURF1 KO-mutant.
Description
Analysis was done using isotopically labeled tracers as described in Spier et al. (2021). "Mitochondrial respiration restricts Listeria monocytogenes infection by slowing down host cell receptor recycling"The data here represent three different experiments refering to Figs. 2A-C in the manuscript.
Regarding the nomenclature of the individual files, Lm refers to Listeria monocytogenes. Subsequently the date of the experiment is mentioned followed by the labeled substrate that was added during cultivation: 13CGlc refers to [U-13C6]glucose, 13CGal refers to [U-13C6]galactose, 13C5Glu refers to [U-13C5]glutamine. Ui means uninfected and the subsequent number indicates biological replicate 1 or 2.
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The final abbreviation refers to the metabolites under analysis in the respective expirement: AS meaning protein-bound amino acids and PM meaning polar metabolites extracted from the cytosol.
Links
Where it is published
- DOI doi.org/10.11922/sciencedb.997 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.11922%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Medicine & Health · Social Science
- Inferred from text
- Biochemistry and cell biology 74%
Provenance · 1 source records, 12 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.11922/sciencedb.997 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · scidb cn | taxonomy-embedding@1.0.0 | title+keywords+description (74%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/rights |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |