Data · dataset · 2026
Discovery of an antibacterial peptide QUB-2503 derived from Odorrana schmackeri skin secretion and testing of its antibacterial activity and cytotoxicity
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.17034/32641860.v1
Due to the widespread use of antibiotics, bacterial resistance has become a huge challenge in human society.
Description
Therefore, it is important to find alternative treatment methods. In this process, the excellent antibacterial effects and low side effects of natural antimicrobial peptides (AMPs), have attracted the attention of researchers.
At the same time, natural AMPs are widely present in nature. Therefore, extracting and studying natural AMPs is of great significance for the development of human society. In this thesis, gentle electrical stimulation was used to extract skin secretions from Odorlana schmackeri from which a new bioactive peptide was cloned, sequenced and named, QUB-2503.
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Through sequence comparison, it was confirmed that QUB-2503 belongs to the Brevinin-1 family. The peptide was successfully synthesised using solid-phase peptide synthesis, then purified, identified and isolated using RP-HPLC and MALDI-TOF MS. Finally, the antibacterial activity and cytotoxicity of the purified peptide were tested.
QUB-2503 has a stable biological structure, is easy to synthesize and purify, and has good water solubility. In addition, QUB-2503 has good antibacterial activity against Gram positive Staphylococcus aureus, with a MIC of 32 μM. Meanwhile, QUB-2503 has low cytotoxicity towards red blood cells (HC50＞256 μM) and normal lung cells (IC50 of MRC-5＞100 μM), indicating good safety.QUB-2503 is thus a bioactive peptide with potential medical value.<br><br><i>Thesis is embargoed until 31 December 2030.</i>
Links
Where it is published
- DOI doi.org/10.17034/32641860.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
Provenance · 3 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/32641860 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/32641860 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/32641860 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |