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Omics · study · 2026

Effect of depletion of Nsd1 or Ezh2 on gene expression during pluripotency transition

Listed in NCBI GEO

CRISPR Screen with single-cell RNA-Seq as readout reveals new epigenetic factors regulate pluripotency of m ESCs.

Description

This highly sensitive method identified key genes essential for the acquisition and exit from pluripotency, and revealed loss of Nsd1-mediated H3K36me2 delayed exit from the naive state, while loss of Ezh2-mediated H3K27me3 promoted transition to the primed state.

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Expression profiling by high throughput sequencing · Mus musculus
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Life Sciences
Inferred from text
RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
SourceKeyLast seenRaw
NCBI GEOGSE33479115 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencingsource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[modality].local:modality:rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (65%)
concepts[modality].local:modality:single-cell-rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (65%)
concepts[organism].NCBITaxon:10090source · NCBI GEOconnector:ncbi_geo@1.0.0/taxon
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title