Text · study · 2026
MeRIP-seq profiling of m⁶A modifications in Alkbh5-KO and control bone marrow-derived macrophages following M1 polarization
Listed in NCBI GEO
We performed MeRIP-seq combined with RNA-seq to identify downstream targets of the m⁶A demethylase ALKBH5 in macrophages.
Description
Bone marrow-derived macrophages from myeloid-specific Alkbh5 knockout and Alkbh5-fl/fl littermate control mice were polarized to the M1 phenotype with LPS and IFN-γ. m⁶A-enriched RNA was immunoprecipitated using an anti-m⁶A antibody and sequenced on an Illumina NovaSeq 6000 platform. Altered m⁶A peaks in Alkbh5-KO cells were predominantly located within coding sequences, 3′ untranslated regions, and regions near stop codons.
Integrative analysis with RNA-seq identified genes with both altered m⁶A methylation and differential expression, revealing the m⁶A epitranscriptomic landscape regulated by ALKBH5 in M1-polarized macrophages.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337112 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337112 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1484068 ncbi.nlm.nih.gov/bioproject/PRJNA1484068 ↗
project · from NCBI GEO
Topics
- Stated by source
- Mus musculus · Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337112 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |