Data · dataset · 2026
<p>MARCHF5 is required for survival of PEL cell lines.</p>
Listed in UCL Research Data Repository
<p>A. Cumulative live cell counts of <i>MARCHF5</i> Cas9 knockout (KO) cells.
Description
To start the growth curves, BC-3 cells were seeded at 3 x 10<sup>5</sup> cells/mL 3-4 days after transduction with lentiviruses expressing both Cas9 and the targeting gRNA against <i>AAVS1</i> or <i>MARCHF5</i>. BCBL-1 iCas9 cells expressing the gRNAs were similarly seeded in triplicates and Cas9 expression was induced by Doxycycline (Dox) treatment (0.5 μg/mL).
Every three days, total live cells were counted by trypan blue exclusion assay and split back to 3 x 10<sup>5</sup> cells/mL to allow the cells to continue proliferating. Cumulative live cell counts are expressed relative to the negative control gRNA <i>AAVS1</i>. B. Representative Western blots of MARCHF5 from cells harvested at Day 6 from similar experiments from panel A. C. Quantification of MARCHF5 Western blots normalized to Revert Total Protein stain signal from MARCHF5 KO cells from experiments collected for this study.
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For panels A and C, statistical differences were calculated using two-way ANOVA with Tukey’s multiple comparison post-hoc test. Adjusted <i>p</i> values reflect post-hoc comparisons between g<i>AAVS1</i> to either <i>MARCHF5</i> gRNA at the specific time point. Error bars, standard error of the mean.
D. MARCHF5 CRISPR Scores (Gene Effect Score) from genome-wide CRISPR screens in non-cancerous control immortalized cell lines (5 prostate cell lines, 5 retinal pigment epithelial cells, and 2 B cell lines) as well as 3 PEL cell lines were downloaded from DepMap 26Q1. As reference points, a Gene Effect Score of 0 (gray line) denotes that a gene does not affect fitness of a specific cell line while a Gene Effect Score (red line) of 1 represents the median fitness score for pan-essential genes (“housekeeping”) defined by DepMap.
The box plots and whiskers represent the median and lower and upper limits of each group.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014665.g002 ↗
DOI / persistent id · from rdr ucl ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from rdr ucl ac uk
Topics
- From keywords
- Biochemistry and cell biology · Cancer · Earth & Environmental Science · Genetics · Infectious diseases · Life Sciences · Medicine & Health · Virology
Provenance · 1 source records, 13 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| UCL Research Data Repository | oai:figshare.com:article/34049120 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[disease].local:disease:cancer | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[field].anzsrc:field:310706 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| description | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| title | source · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | /metadata/dc/title |