Omics · study · 2026
Cutaneous SCFAs are reduced in infants with atopic dermatitis, and the SCFA-sensing receptor GPR43 modulates skin barrier integrity and inflammation
Listed in NCBI GEO
Description
Background
Early-life skin colonization by commensal microbes is associated with reduced risk of atopic dermatitis (AD), but underlying mechanisms remain unclear. Short-chain fatty acids (SCFAs), gut microbiome-derived metabolites with immunomodulatory functions, are also produced by cutaneous commensals, yet the roles of cutaneous SCFAs and their sensing receptor GPR43 in AD remain unclear.
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Methods
SCFA levels were quantified in non-lesional skin of infants with AD and controls. GPR43 expression was analyzed in flaky tail (Flgft) mice and human AD skin. The functional effects of GPR43 activation were evaluated in human keratinocytes and in vivo in Flgft mice using a GPR43 agonist.
Results: Propionate and butyrate levels were reduced in non-lesional skin of infantile AD. Epidermal GPR43 expression was decreased in early life in Flgft mice. In keratinocytes, GPR43 was upregulated by differentiation, SCFAs, and type 2 cytokines.
GPR43 activation promoted keratinocyte differentiation and long-chain ceramide synthesis, and attenuated IL-4/IL-13–induced barrier dysfunction and TSLP expression. Topical GPR43 agonist improved barrier function and reduced dermatitis in Flgft mice. Conclusion: Dysregulation of the SCFA–GPR43 axis occurs in the skin during early life in AD.
Cutaneous GPR43 activation enhances barrier integrity and suppresses inflammation, suggesting a potential therapeutic strategy in AD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336219 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336219 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1481098 ncbi.nlm.nih.gov/bioproject/PRJNA1481098 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336219 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |