Response to varying phosphorus application in perennial forages at an Ontario silt-loam site: Supplementary data

In the third year, samples of roots were taken from monoculture plots and assessed for arbuscular mycorrhizal fungi. In the fall of 2024 (after the final harvest of the trial) samples of the plots were subjected to a simulated freeze-thaw experiment to assess potential for P loss from the plots over winter. Soil and tissue testing was conducted by SGS Canada's facility in Guelph Ontario.

Water Extractable P (WEP) analysis was conducted at Agriculture and Food Laboratory at the University of Guelph. For a further explanation of methods, please see the MSc thesis "Response to varying phosphorus application in perennial forages at an Ontario silt-loam site", Chapters 2.3 (P fertility trial methods) and 3.3 (freeze-thaw experiment methods).