Mechanostimulation data of ApPr of CSF-cN

Juego de datos de electrofisiología para construir las Figuras 6D y 6E del manuscrito "A filopodia-based dendritic mechanosensory compartment in CSF-contacting neurons" doi.org/10.64898/2026.04.09.713694. The details of the analysis are explained in the “METHOD DETAILS” section of the above mentioned article. Briefly, we prepared transverse or 45°-angled lumbar spinal cord slices (300 µm thick) from GATA-3 eGFP mice and performed patch-clamp recordings from CSF-cNs.

We used the voltage-clamp configuration to record mechanically evoked currents, and the cell-attached mode to assess the impact of these stimuli on neuronal excitability. For mechanical stimulation we positioned a pipette with Luigs & Neumann micromanipulators to stimulate the ApPr or soma with a 2 µm (or 4 µm) 'step' producing an indentation (push) on the desired compartment. We acquired data at 20 kHz (low-pass filtered at 2.9 kHz) using a Heka Elektronik EPC 10 USB Patch Clamp Amplifier (RRID:SCR_018399) and Patchmaster software (RRID:SCR_000034).