Study of the yeast cytosolic Hsp70-system in protein homeostasis and life span regulation - Silver stained 2D-gels of protein extracts from wild type and Hsp70-mutant yeast strains

To answer this question, we utilised the disparate functions of the Hsp70's Ssa1 and 2 and their paralog Ssa4 in a yeast strain that lacks Ssa1/2 but has an ectopically increased production of Ssa4. We have gathered data on the behaviour of several different markers for protein aggregation under different circumstances, as well as data on proteins from other classes of molecular chaperones.

The bulk of the data is in the form of multichannel microscopy images from widefield microscopy, with a few sets of western blots of protein extracts. Images of two dimensional protein gels with protein extracts from wild type and Hsp70 mutant yeast strains in mid-exponential growth phase. The yeast were grown in rich medium (YP) with 2 % glucose as carbon source.