Data · dataset · 2026
<p>LPS-induced apoptosis and epithelial barrier disruption in pSIOs.</p>
Listed in Deakin Research Online and DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.1371/journal.pone.0359086.g003
<p>(A) Phase-contrast images of pSIOs treated with LPS (0, 20, and 30 μM) showing structural disruption, core darkening, and fragmentation at higher concentrations compared with controls.
Description
Scale bars: 200 μm. (B) DAPI (blue) and PI (red) staining of pSIOs treated with LPS (5, 10, 20, and 30 μM) showing a dose-dependent increase in apoptosis.
Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each LPS concentration in each biological replicate. Scale bars: 200 μm. (C) Representative immunofluorescence images of E-cadherin (green) and DAPI (blue) staining of pSIOs showing reduced and discontinuous E-cadherin expression in LPS-treated organoids compared with controls.
Read the rest (2 more)
Enlarged views of the yellow boxed regions are shown. Low-magnification (200 μm) images and enlarged views (50 μm) are shown in 3C. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate.
(D) Relative gene expression levels of <i>CDH1</i> and <i>TJP1</i> in pSIOs treated with LPS (20 μM) compared with controls (0 μM), as determined by qRT-PCR. LPS treatment significantly decreased the expression of <i>CDH1</i> and <i>TJP1</i>, indicating disruption of epithelial barrier integrity. Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. ***<i>p</i> < 0.001.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0359086.g003 ↗
DOI / persistent id · from dro deakin edu au
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from dro deakin edu au
Topics
- From keywords
- Biochemistry and cell biology · Biochemistry and cell biology · Biochemistry and cell biology · Chemistry · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Immunology · Immunology · Immunology · Infectious diseases · Infectious diseases · Infectious diseases · Life Sciences · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health · Medicine & Health · Microbiology · Microbiology · Microbiology · Research, science and technology policy · Research, science and technology policy · Research, science and technology policy
- Inferred from text
- Image 65%
Provenance · 3 source records, 36 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Deakin Research Online | oai:figshare.com:article/34048150 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34048150 | 13 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/34048150 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].anzsrc:field:320211 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:320211 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:440710 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:field:440710 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:field:440710 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:group:3101 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:chemistry | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · dro deakin edu au | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | /metadata/dc/description |
| license | source · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | /metadata/dc/rights |
| publication_date | source · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| title | source · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | /metadata/dc/title |