Data Sheet 1_Interleaving SLIDE microscopy.pdf

However, imaging speeds have traditionally been limited because images are generated by point scanning. Recently, Spectro-Temporal Laser Imaging by Diffractive Excitation (SLIDE) introduced a high-speed laser-scanning approach for two-photon microscopy, harnessing a swept-source laser in combination with a diffraction grating for inertia-free point scanning with line-rates at Megahertz (MHz) frequencies and frame-rates in the kilohertz range.

Notably, the pixel-rate reached up to Gigapixels/s, which scales with ultra-short second harmonic generation signals. However, this nanosecond pixel dwell time is too short for fluorescence decays. Here, we present an adaptive approach to high-speed multiphoton imaging where the pixel-rate of the SLIDE microscope can be adapted to the fluorescence lifetime of the sample.