Data · dataset · 2026
<p>Screen for suppressors of the <i>orc4-R267A</i> lethal phenotype.</p>
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1371/journal.pone.0349465.g001
<p>A) An <i>orc4-R267A</i> strain kept alive by a plasmid containing <i>ORC4</i> was treated with ethyl methane sulfonate (EMS) to isolate suppressors of the lethal phenotype of the <i>orc4-R267A</i> allele.
Description
Only strains that acquire suppressors can lose the plasmid. The desired strains turn red and grow on 5-Fluorouracil (5-FOA) due to the loss of the <i>ADE2</i> and <i>URA3</i> genes on the plasmid.
B) Table of <i>orc4-R267A</i> suppressor mutations identified in the screen. C) Ten-fold serial dilutions of strains that have <i>ORC4</i> on a counter-selectable plasmid and either <i>ORC4</i> or <i>orc4-R267A</i> allele integrated into the genome. The presence of suppressors-of-orc-four-ATPase alleles are indicated.
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Some strains are red prior to 5-FOA selection because the suppressor allele allowed plasmid loss during strain construction.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0349465.g001 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Biochemistry and cell biology · Biochemistry and cell biology · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Genetics · Life Sciences · Life Sciences · Life Sciences · Saccharomyces cerevisiae · Saccharomyces cerevisiae · Saccharomyces cerevisiae
- Inferred from text
- Tabular 65%
Provenance · 3 source records, 21 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34035570 | 13 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34035570 | 13 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34035570 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:tabular | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:4932 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| concepts[organism].NCBITaxon:4932 | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['saccharomyces cerevisiae </'] |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |