Data · dataset · 2026
<p>Sph targets Mic60 and disrupts MICOS organization and mitochondrial cristae homeostasis.</p>
Listed in ZivaHub
<p><b>(A-C)</b> Co-immunoprecipitation of His-Mic60<sub>1-590</sub> with eGFP-Sph, eGFP-T<sub>149</sub>A, or eGFP-H<sub>150</sub>N in co-transfected NIH/3T3 cells using an anti-His antibody. <b>(D)</b> Schematic representation of Mic60 and the truncated Mic60 constructs used for domain-mapping analyses.
Description
(E-I) Co-immunoprecipitation analysis of eGFP-Sph with His-Mic60<sub>1-590</sub>, His-Mic60<sub>35-480</sub>, His-Mic60<sub>35-200</sub>, His-Mic60<sub>201-327</sub>, or His-Mic60<sub>328-590</sub> in co-transfected NIH/3T3 cells using an anti-GFP antibody. <b>(J)</b> Representative fluorescence images showing mitochondrial localization of eGFP-Sph in NIH/3T3 and HEK-293T cells.
Scale bars, 10 μm. <b>(K)</b> Immunoprecipitation of endogenous Mic60 from HC11 cells treated with recombinant Sph (32 μg/mL) for 6 h using an anti-Mic60 antibody. <b>(L, M)</b> Co-immunoprecipitation analysis of Mic60 association with Mic19 (L) or Mic10 (M) in HC11 cells following recombinant Sph treatment (32 μg/mL, 6 h), using anti-Mic19 or anti-Mic10 antibodies, respectively. <b>(N)</b> Representative immunoblot showing the relative abundance of long and short OPA1 isoforms in HC11 cells treated with recombinant Sph (32 μg/mL) for 6 h.
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Data are presented as mean ± SD. **<i>P</i> < 0.01.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.ppat.1014644.g005 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Biochemistry and cell biology · Earth & Environmental Science · Genetics · Immunology · Infectious diseases · Life Sciences · Medicine & Health · Microbiology · Research, science and technology policy · Virology
- Inferred from text
- Image 65%
Provenance · 1 source records, 16 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34026600 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:field:310706 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Virology'] |
| concepts[field].anzsrc:field:320211 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:440710 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:group:3101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |