miRNA expression profiles in extrracellular vesicles released from mouse CD4+ T cells

It is known that miRNAs encapsulated within these extracellular vesicles (EVs) are involved in the regulation of cellular function and the control of disease. It has become increasingly clear that secretory PLA2 (sPLA2), an extracellular metabolic enzyme, metabolizes the membrane lipids of EVs and regulates their function.

Therefore, we focused on PLA2G12A, a member of the sPLA2 family, and investigated whether its deficiency alters the miRNA profile. We isolated naive T cells from mouse spleens, differentiated them into Th17 cells using a Th17 differentiation culture system, and analyzed the EVs released during this process. Although no significant overall differences were observed between wild-type and gene-deficient, differences in expression levels were confirmed for specific miRNAs.