Age-related gene expression alteration responding to mechanical force and TGFβ signaling [ATAC-seq]

These changes are pivotal in modulating cellular responses to mechanical and biochemical stimuli. This study investigates the hypothesis that aging leads to fibroblasts’ divergent adaptation mechanisms to external stimuli.

Herein, fibroblasts from young and old cohorts were embedded in 3D collagen gels and subjected to tension and Transforming Growth Factor beta (TGFb) stimulation. The study revealed that mechanical forces and aging exert profound effects on chromatin architecture. Young cells adapted more rapidly to mechanical stress and showed a heightened responsiveness to TGFβ compared to older cells.