A follow-up study on the influence of genetics and environment on adolescent depression

Process：The first measurement was in May 2021, measuring the independent variable (peer-student relationship and teacher-student relationship) with the baseline period dependent variable (adolescent depression), with uniformly trained psychology faculty and graduate students as the main testers, ensuring the cooperation of two main testers per class, using the same instructors and uniform time points, with classroom teachers on hand to assist with the survey.

The first follow-up was in November 2021 to measure the mediating variable (frustration), and the questionnaire measurement process was the same as the first measurement. As this required genetic collection (oral epithelial cells), classroom teachers were entrusted to inform students before collection that each student should use water to rinse their mouth 30 min before collection, after which they should not eat or drink anything that would affect the purity of the sample.