Data from: Integrating morphological characters, molecular markers, and distribution patterns to assess the identity of Blepharis species from Jordan

Results: Shoots from 19 randomly chosen Blepharis plants per population were harvested for morphological and molecular analyses. Spikes were lax and bract width was considerably shorter than length of the longest lateral spine in the Northern populations compared to the Southern population (P < 0.001). A multivariate linear discriminant analysis (LDA) distinguished the Northern populations from the Southern one largely by internode length, bract width, longest lateral spine length, and bract width to spine length ratio (P < 0.001).

The ISSR analysis revealed that 44 markers across eight primers were polymorphic with major allele frequency of 83.6% and an average of 5.5 polymorphic markers per primer. The genetic resemblance among individuals ranged from 0.27 to 0.96. The three Blepharis populations were accordingly clustered into two distinct groups, similar to the analysis of morphological differences.

Conclusions: Our results strongly indicate the occurrence of two discrete Blepharis species in Jordan and the hypothesis that the genus is represented by only one species is not supported. The species in Jordan are B. attenutata Napper (represented by the Northern populations) and B. ciliaris [L.] B. L. Burtt (represented by the Southern population). These findings are important for informing floristic work within the region and an updated key has been included in our findings.

Usage notes

Morphological Data Morphological data (see README.md for variable description). All data & analysis files available as an .Rproj project file from GitHub: github.com/ColauttiLab/BlepharisMorphData MuhaidatEtAl_MorphData.csv LDA.R R code for Linear Discriminant Analysis LDAvsISSR R code for comparing LDA with NMDS from ISSR genotype data MuhaidatEtAl_ISSRData ISSR genotype data