Omics · study · 2026
Nootkatone preferentially suppressed the AKT/mTOR signaling in activated fibroblasts
Listed in NCBI GEO
Having established that nootkatone restores autophagic flux and that autophagy contributes functionally to its anti-fibrotic activity, we next sought to identify the upstream events associated with this response.
Description
Transcriptomic profiling was therefore performed in TGF-β1–activated lung fibroblasts treated with or without nootkatone. RNA-seq analysis revealed clear separation among control, TGF-β1-treated, and nootkatone + TGF-β1-treated fibroblasts by principal component analysis, indicating distinct transcriptional states across groups。Pathway enrichment analysis of genes downregulated by nootkatone revealed significant enrichment of PI3K-Akt signaling and focal adhesion–PI3K-Akt-mTOR signaling, together with matrix-adhesion and cytoskeletal programs, including ECM-receptor interaction, focal adhesion, and cytoskeleton in muscle cells
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336865 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336865 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1483477 ncbi.nlm.nih.gov/bioproject/PRJNA1483477 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336865 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |