<b><i>Citrus tristeza virus</i></b><b> (CTV): 3D Virion Architecture, Vector Transmission Biophysics (EPG), and Field Diagnostics</b>

<p dir="ltr">This audiovisual documentary and scientific visualization covers the structural biology, transmission dynamics, and field diagnostics of <i>Citrus tristeza virus </i>(CTV, genus <i>Closterovirus</i>).</p><p dir="ltr"><b>Key Modules Featured:</b></p><ol><li><b>Virion Architecture</b>: 2000 nm flexuous rod showcasing the major capsid protein (CP/p25) covering ~95% of the genome and the minor capsid protein (CPm/p27) forming the 5' rattle snake-like terminal structure.</li><li><b>Comparative Genomics</b>: Alignment track analyzing divergence across virulent quick-decline (VT), severe stem-pitting (T36), mild cross-protecting (T30), and Indian acid lime (Kpg) isolates in p33, p20, and 5' UTR regions.</li><li><b>Vector Transmission Biophysics</b>: Real-time Electrical Penetration Graph (DC-EPG) waveforms representing intercellular pathway (waveform C), intracellular potential drops (pd), phloem salivation/inoculation (E1), and phloem sap ingestion/acquisition (E2) by the brown citrus aphid (<i>Toxoptera citricida</i>).</li><li><b>Rootstock Incompatibility Risk</b>: Cambial necrosis analysis comparing Sweet Orange on Sour Orange rootstocks against tolerant Rough Lemon and Rangpur Lime cultivars.</li><li><b>Rapid Field Diagnostics</b>: Conserved CP/p25 isothermal amplification (RT-LAMP) at 65°C with colorimetric visual dye indicators (HNB and Phenol Red) coupled with lateral flow dipstick chromatography.</li></ol><p></p>