Liver samples collected for investigations of enzyme activity of Glutathione-s-transferase in the Antarctic fish Dissostichus mawsoni and Pagothenia borchgrevinki

The second phase involves the thermodynamic characterization of enzymes from D. mawsoni being compared with those of other organisms with different temperature regimes (i.e. moth Galleria mellonella and a rat). The third phase involved the characterization of enzyme levels with different substrates. In particular endogenous xenobiotic substrates were examined.

To summarize, the first phase examined the need for a detoxication system. The second examines any structural evolutionary change unique to the Antarctic and the third examines specialization of enzyme types that may have occurred to facilitate life in Antarctic waters.