Data Sheet 1_An unfolded protein response-inducible protein (ULI1) mediates aromatic alcohols biosynthesis through reinforcing the Ehrlich pathway in Saccharomyces cerevisiae.zip

Compared with the control KMLY1-6, the CIE strains increased 2-PE and TOL production by 10.13–12.91% and 25.76–29.28%, respectively. Meanwhile, the PBE strain produced significantly higher levels of 2-PE and TOL than the empty vector control (BY4741/pY26), with maximum increases of 38.80% and 30.86% detected at 60 h of cultivation. To further elucidate the molecular mechanism of ULI1 in 2-PE and TOL biosynthesis, comparative transcriptomic analysis for Kmuli1 vs. KMLY1-6, together with transcriptomic and proteomic analyses for BY4741/pY26-uli1 vs. BY4741/pY26, was performed.

Multi-omics results revealed that ULI1 reinforces the Ehrlich pathway, modulates metabolic flux, maintains mitochondrial structure and cellular protein homeostasis, and coordinates stress responses with metabolic redistribution. Collectively, ULI1 acts as a novel regulatory hub linking stress tolerance and cellular homeostasis, and facilitates aromatic alcohol biosynthesis by strengthening the Ehrlich pathway in S. cerevisiae.</p>