Imaging · study · 2026
Spatial transcriptomic mapping of canine osteosarcoma reveals immune microenvironment features linked to survival
Listed in NCBI GEO
Osteosarcoma (OSA) is the most common malignant bone tumor in dogs and serves as a translational model for human pediatric OSA due to shared molecular features.
Description
This aggressive and metastatic cancer exhibits significant heterogeneity, which has limited therapeutic advances. Spatial transcriptomics offers a powerful method to investigate intratumoral complexity and the tumor microenvironment.
In this study, we used the 10x Genomics Visium platform to profile spatial gene expression in 14 fresh-frozen canine OSA (cOSA) tumor samples from 11 dogs, including primary, metastatic, and recurrent tumors. Samples were stratified by survival outcomes (short-, median-, and long-term). Spatially variable genes and conserved transcriptional clusters were identified, and integration with published single-cell cOSA data enabled deconvolution of cell type proportions and spatial co-localization.
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Analysis of 18,834 tissue spots revealed intra-tumoral subpopulations and spatial clustering patterns. Pathways related to immune clearance were spatially enriched in long-term survivors. Deconvolution uncovered co-localization of immune cells such as mast cells with osteoclasts, and T/NK cells with regulatory dendritic cells.
This study maps the spatial transcriptomic landscape of cOSA, highlighting immune microenvironment features and spatial gene expression patterns associated with survival. These findings provide insights into OSA biology and may guide future therapeutic strategies in both canine and human patients.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299494 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299494 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1274825 ncbi.nlm.nih.gov/bioproject/PRJNA1274825 ↗
project · from NCBI GEO
- PubMed 42056464 pubmed.ncbi.nlm.nih.gov/42056464 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Other
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75%
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE299494 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |