Excel · study · 2026
Intravitreal delivery of the cationic micelle DMP suppressed the development of choroidal neovascularization
Listed in NCBI GEO
Age-related macular degeneration (AMD)-associated choroidal neovascularization (CNV) causes severe vision loss.
Description
This study evaluated the therapeutic potential of cationic DMP micelles for CNV. DMP micelles were synthesized by modifying PEG-PCL with DOTAP, to form self-assembled nanoparticles.
Cytotoxicity was assessed in 661w and human Müller cells via the MTT assay. BN rats with laser-induced CNV received intravitreal DMP or saline. CNV severity was evaluated via fundus fluorescein angiography (FFA) and IB4-stained neovascular area quantification.
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Retinal transcriptomes were analyzed by RNA-seq. Cellular uptake mechanisms were studied via the use of endocytosis inhibitors (chlorpromazine, methyl-β-cyclodextrin, filipin III). Endothelial migration was tested via a scratch assay.
DMP micelles exhibited favorable properties (86.48 nm, +46.4 mV) and low cytotoxicity (IC50 >300 mg/mL vs PEI25K). DMP significantly reduced the FFA leakage score (1.2±0.4 vs saline 2.5±0.6, p<0.01) and IB4+ area (35,602±5,412 vs 58,341±7,203 μm², p<0.001). RNA-seq revealed upregulated antigen presenting genes (RT1-Da, RT1-Ba, RT1-Db1, RT1-Bb) and the immune modulator Slpi.
Cellular uptake involved clathrin- and lipid raft-mediated endocytosis. DMP inhibited endothelial migration in vitro. DMP micelles demonstrate dual antiangiogenic and immune-modulating effects in CNV models with retinal biocompatibility.
The ability of DMP to suppress neovascularization and activate antigen presentation pathways highlights their therapeutic potential for wet AMD.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297943 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297943 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1267001 ncbi.nlm.nih.gov/bioproject/PRJNA1267001 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE297943 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |