<p>Subclinical cases display IgG-SC of higher affinity for DENV1 sE and sE-dimer, and higher frequencies of serotype cross-reactive IgG-SC compared to hospitalized patients.</p>

Horizontal bars indicate median and interquartile range. Each dot represents one sE-protein-specific IgG-SC. <i>p</i> values were calculated using Kruskall-Wallis followed by Dunn’s multiple comparison test. ns, not significant; *, p ≤ 0.05; **, p ≤ 0.01.

The dotted line indicates the 30nM affinity limit that discriminates E protein binders from non-binders. <b>B.</b> Proportions of IgG-SC binding to the different sE and/or sE-dimer proteins in both patient groups. Number of patients (n), total number of anti-E protein IgG-SC analyzed and percentages of anti-E protein IgG-SC falling in each category are indicated. <b>C.</b> (Top) Frequencies of IgG-SC binding to sE-dimer of only one serotype or both serotypes in subclinical infected cases.