The difference of gene expression and splicing between the WT and scl mutants.

We here report the SR gene as a key regulator of gene expression and alternative splicing in rice (Oryza sativa). Comparative RNA-seq analysis between wild-type and scl mutant plants revealed significant differential expression of genes involved in growth, development pathways.

Notably, the sr mutation resulted in widespread alterations in alternative splicing patterns, particularly affecting genes involved in signal transduction and transcriptional regulation. Although the sr mutation did not affect the binding of general transcription factors to promoter regions, it specifically impaired the coordination between transcription and splicing machinery for a subset of genes with critical developmental functions.