Discovery of two natural strains closely similar to the model strain Anabaena sp. PCC 7120 used in molecular studies of heterocyst differentiation

Fig.S1 Comparison of gamma plasmids (a) or delta plasmids (b) from Anabaena strains FACHB-595, FACHB-709 and PCC 7120 Fig.S2 Electrophoretograms of PCR products showing the segregation of mutants. a to k, PCR examination of hetR, hetP, hetZ, patS, patU3, patX, patL, patA, patN, nrrA and hetC mutants Each mutant was examined with two pairs of primers: the first pair, with one primer located within the deleted region and the other primer outside the deleted region (lanes 1, 3, 5 and 7); the second pair, with both primers outside the deleted region (lanes 2, 4, 6 and 8).

Lanes 1-2, the deletion mutant of FACHB-595; lanes 3-4, the wild type of FACHB-595; lanes 5-6, the deletion mutant of FACHB-709; lanes 7-8, the wild type of FACHB-709; M, marker. The Trans 5K marker (0.3, 0.5, 0.8, 1, 1.5, 2, 3, 5 kb) was used in a, d, e, g, i and k, while that used in other electrophoretograms was the 100-bp marker (0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.2, 1.5 kb). Table S1 List of strains, plasmids and primers used in this study