<p>The percentage of variants above folding free energy thresholds.</p>

<p>Panel A shows the number (left axis, solid lines) and fraction (right axis, dashed lines) of labeled variants that are pathogenic (containing likely pathogenic and pathogenic) and benign (containing likely benign and benign) with a predicted value above the threshold defined by the X-axis.Panel B focuses on the number (left axis, solid lines) and fraction (right axis, dashed lines) of VUS that reach either the strong posterior (0.6) or very strong posterior (0.98) with a value above the threshold defined by the X-axis.

Panel C plots the percentage of variants that with value above a series of thresholds for labeled variants and for the VUS above the posterior of 0.98. Panel D shows the genes where at least 50% of their LP/P variants have a of at least 1 kcal/mol. Genes with less than 50% of their pathogenic variants above the threshold are included in Fig E in the <a href="plosgenetics.org/article/info:doi/10.1371/journal.pgen.1012085#pgen.1012085.s001" target="_blank">S1 Appendix</a>.