Omics · study · 2026
Fibrotic Remodeling in the NOD/ShiLtJ Mouse Model of Sjögren’s Disease: Insights from Single-Cell Transcriptomics and AI-Driven Quantification
Listed in NCBI GEO
Sjögren’s Disease (SjD) is a systemic autoimmune disorder characterized by salivary gland hypofunction and lymphocytic infiltration, yet the contribution of fibrosis to glandular dysfunction remains unclear.
Description
The NOD/ShiltJ mouse is a well-established model for SjD. In this study, we evaluated the presence of and impact of fibrosis in the salivary glands of the NOD/ShiLtJ mouse.
Using scRNASeq, we investigated differential gene expression in the fibroblasts of NOD mouse vs control mice. Fibroblast populations in the salivary glands of the NOD mouse exhibited increased levels of extracellular matrix mRNAs, including Col1a1, Col1a2, and Col3a1, relative to the control strain. From histological staining with Picrosirius red and AI-driven fibrosis quantification, we identified significant fibrotic remodeling in the submandibular glands of non-obese diabetic NOD/ShiLtJ mice relative to controls, which correlated with the diabetic phenotype in this mouse strain.
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We assessed the therapeutic potential of Nintedanib to alleviate this fibrotic phenotype. Nintedanib is an FDA-approved antifibrotic agent for chromic fibrosing interstitial lung disease. While Nintedanib did not significantly reduce immune infiltration or restore salivary function, it modestly decreased multiple fibrosis scores in long-term treatment cohorts.
These findings demonstrate that the NOD/ShiLtJ strain models the fibrotic response that occurs in SjD patients and supports further investigation into antifibrotic therapies for salivary gland dysfunction.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE314nnn/GSE314772 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE314772 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1392576 ncbi.nlm.nih.gov/bioproject/PRJNA1392576 ↗
project · from NCBI GEO
- PubMed 42548660 pubmed.ncbi.nlm.nih.gov/42548660 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Disease 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE314772 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |