Table · dataset · 2026
Supplemental Material For: Mesenchymal stromal cell derived membrane particles improve metabolic activity and functional performance during porcine kidney normothermic machine perfusion
Listed in HKU DataHub and figshare and Loughborough Research Repository — shown once because both records carry DOI 10.6084/m9.figshare.34002339.v1
<p dir="ltr"><b>Introduction</b></p><p dir="ltr">Kidneys from donation after circulatory death (DCD) donors are increasingly used to alleviate organ shortage but highly vulnerable to ischemia-reperfusion injury, leading to poor graft outcomes.
Description
Normothermic machine perfusion (NMP) enables targeted intervention during donor kidney preservation. Membrane particles (MPs) derived from mesenchymal stromal cells (MSCs) have shown regenerative potential.
We aimed to investigate MPs as a potential therapeutic strategy to improve metabolism and function in porcine DCD kidneys during NMP.</p><p dir="ltr"><b>Methods</b></p><p dir="ltr">Six pairs of porcine kidneys underwent 4-hour NMP. Within each pair, one kidney received MPs derived from 1.0×10<sup>7</sup>MSCs isolated from the abdominal subcutaneous adipose tissue of healthy donors, while the contralateral kidney served as a control.
Read the rest (3 more)
Hemodynamics, sodium reabsorption, oxygen consumption, and injury markers were monitored. Gene expression related to inflammation and metabolism were analyzed.</p><p dir="ltr"><b>Results</b></p><p dir="ltr">MP administration did not affect renal blood flow or intrarenal resistance. Injury markers (AST, LDH,<i>IL-6</i>) were increased over time in both groups, without significant difference between MP-treated and control kidneys.
In contrast, MP-treated kidneys showed significantly higher expression of metabolic genes (<i>PDHB</i>,<i>ATP6V0D2</i>,<i>NDUFS8</i>). Functionally, MP administration enhanced sodium reabsorption, urinary creatinine excretion, and oxygen utilization efficiency compared with controls.</p><p dir="ltr"><b>Conclusion</b></p><p dir="ltr">MSC-derived MPs can be safely delivered to porcine kidneys and promote kidney functional performance and metabolic activity.
These findings support MPs as a promising cell-free therapeutic strategy to improve the quality of kidneys during NMP.</p><p dir="ltr"><br><br></p>
Links
Where it is published
- DOI doi.org/10.6084/m9.figshare.34002339.v1 ↗
DOI / persistent id · from datahub hku hk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from datahub hku hk
Topics
- From keywords
- Astronomy & Astrophysics · Chemistry · Chemistry · Computer Science & AI · Computer Science & AI · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Economics & Finance · Economics & Finance · Engineering · Engineering · Humanities · Humanities · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Psychology & Behavioral Science · Social Science · Social Science
- Inferred from text
- Medical biotechnology 69%
Provenance · 3 source records, 31 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| HKU DataHub | oai:figshare.com:article/34002339 | 13 d ago | JSON v1 |
| figshare | oai:figshare.com:article/34002339 | 12 d ago | JSON v1 |
| Loughborough Research Repository | oai:figshare.com:article/34002339 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].anzsrc:group:3206 | enrichment · datahub hku hk | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:materials-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:mathematics-statistics | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| description | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/description |
| license | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/rights |
| publication_date | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| title | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/title |