Effect of miP-PSTPIP2 overexpression on endothelial gene expression in homeostasis and inflammation. [ext501]

Here we report a high-throughput proteo-genomic pipeline that provides cumulative evidence for a large number of bona fide miPs in human and murine endothelial cells. Vascular inflammation in vitro and in vivo profoundly altered smORF expression and a large number of miPs was identified in the blood stream of patients following heart tissue damage.

Human miPs presented high homology with other primates and their functional perturbation identified miPs that were essential for endothelial cell growth and viability. Moreover, combining gain/loss-of-function with multi-omic approaches demonstrated the involvement of an inflammation-regulated miP in the regulation of gene expression, proliferation and inflammation through interaction with proteins in different subcellular compartments.