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Data · dataset · 2020

Fluorescence microscopy data of CYK1-GFP embryos in early C. elegans embryo actomyosin flow

Listed in CaltechDATA

Microscopy movies to study the dynamics of cortical CYK-1 foci in C. elegans during early actomyosin flow by imaging fluorescence recovery after photo-bleaching.

Description

One-cell C. elegans embryos were obtained from animals grown at room temperature, after 24 hours of feeding with E. coli carrying rga-3 RNAi or L4440 (control) constructs, using a Nikon Spinning Disk Confocal Microscope with an Andor DU-888 camera, 100x oil objective, 50 ms Exposure, ~20 frames per second, and maximum laser intensity.

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Where it is published

Catalogue records · 1

Topics

Stated by source
chiral · cortical flow · formin
Inferred from text
Biochemistry and cell biology 70% · Imaging 75% · Microscopy 75%
Provenance · 1 source records, 17 field assertions
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