Spring Blooms of Sea Ice Algae Along the Western Antarctic Peninsula: Effects of Warming and Freshening on Cell Physiology and Biogeochemical Cycles.

The project will provide student training to college students as well as provide for educational experiences for K-12 school children. There is currently a poor understanding of feedback relationships that exist between the rapidly changing environment in the western Antarctic Peninsula region and sea-ice algal production. The large shifts in temperature and salinity that algae experience during the spring melt affect critical cellular processes, including rates of enzyme-catalyzed reactions involved in photosynthesis and respiration, and the production of stress-protective compounds.

These changes in cellular processes are poorly constrained but can be large and may have impacts on local ecosystem productivity and biogeochemical cycles. In particular, this study will focus on the thermal sensitivity of enzymes and the cycling of compatible solutes and exopolymers used for halo- and cryo-protection, and how they influence primary production and the biogeochemical cycling of carbon and nitrogen. Approaches will include field sampling during spring melt, incubation experiments of natural sea-ice communities under variable temperature and salinity conditions, and controlled manipulation of sea-ice algal species in laboratory culture.

Employment of a range of techniques, from fast repetition rate fluorometry and gross and net photosynthetic measurements to metabolomics and enzyme kinetics, will tease apart the mechanistic effects of temperature and salinity on cell metabolism and primary production with the goal of quantifying how these changes will impact biogeochemical processes along the western Antarctic Peninsula. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.