Data supporting the molecular reconstruction of the Anisakis simplex Ani s 14-containing locus and protein

The reconstructed model comprises 76 exons and 75 introns and encodes a candidate 3,841-aa ShKT-containing cysteine-rich protein whose C-terminal 217-aa region corresponds to canonical Ani s 14. The dataset includes reconstructed transcript, CDS and protein sequences, exon–intron coordinate maps, splice-junction support, proteomic peptide evidence, comparative exon–intron conservation, and evidence supporting the experimentally determined 3′ end.

A historical 3′-RACE product defines a 648-nt post-stop region terminating in a non-templated poly(A) tail, with independent support from LC027371.1 and RNA-seq data. The candidate SL1-containing transcript extends to 12,212 nt up to the experimentally supported cleavage/polyadenylation site, excluding the poly(A) tail. The proposed SL1-containing 5′ end remains qualified because no dedicated full-length 5′-end assay was performed.