Cell cycle exit is required for differentiation and cell fate diversification but not neoblast specialisation in planarians

However, how cell fate acquisition is coordinated with cell cycle progression remains poorly understood. To address this, we generated single-cell RNA-sequencing datasets from cells enriched for 2C or 4C DNA content and from animals subjected to RNAi against the cell cycle regulators cdh1 and h2b.

We found that progenitor populations from multiple lineages contain actively cycling cells enriched in the 4C state. Cell fate acquisition to most cell types is acquired progressively during neoblast differentiation. Comparative analysis of cell cycle regulators confirmed a simplified regulatory repertoire in Schmidtea, supporting a central role for cdh1 in controlling cell cycle exit.