Single cell RNA-seq of young and aged whole lungs following IAV infection

Moreover mTOR signaling pathway were downregulated in Cd4+ cluster of aged lungs. It implies that reduced Cd4+ T cell population during IAV infection in aged mice linked to disrupted mTOR signaling pathway.

In contrast, Gene Ontology (GO) analysis of CD8+ T cells from young and aged mice revealed an upregulation of the p53 pathway in aged CD8+ T cells, suggesting increased DNA damage may further restrict their proliferative potential. Moreover, We report analysis of lung single cell transcriptome in metformintreated aged mice infected with influenza A virus (IAV) for 9 days. Gene set enrichment analysis of Cd4+ and Cd8+ T cells revealed that metformin treatment positively enriched E2F targets, type I interferon (IFN α) response, and oxidative phosphorylation pathways.