Melatonin modulates inflammasome and antioxidant signaling in trophoblasts and decidual macrophages

We profiled transcriptomic responses in JEG-3 trophoblast-like cells and primary human decidual macrophages treated with melatonin at different doses. Cells were exposed to vehicle, 1 nM, or 1000 nM melatonin under defined inflammatory conditions.

RNA-seq libraries were prepared using a strand-specific poly(A)-selected protocol and sequenced on a BGISEQ platform. Differential expression and pathway analyses focused on inflammasome and NRF2-related pathways. These data support a role for melatonin in modulating redox–inflammatory balance in placenta-relevant cell types.