Data · dataset · 2024
Ultrasensitive and Visual Detection of Pseudorabies Virus based on CRISPR-Cas12b
Listed in ScienceDB
Aujeszky's disease (AD) is an acute infectious disease that infects pigs or other animals, resulting in significant economic losses and posing a threat to human health.
Description
Reliable and rapid detection methods are essential for the prevention and control of AD. In this study, a platform based on recombinase aided amplification (RAA) and CRISPR-Cas12b has been established, optimized, and evaluated for the rapid detection of Pseudorabies Virus (PRV).
To enhance the usability and simplicity of the assay, we have developed PRV RAA-Cas12b methods for fluorescence-based detection and visualization. There was no cross-reaction with PRV Bartha-K61 strain or other common swine infectious viruses. The analytical sensitivity of the assay was determined to be 15 copies/µL by real-time fluorescence read-out with a 95% probability.
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The limit of detection (LOD) for the visual PRV RAA-Cas12b assay was 140 copies/µL with a 95% probability. Thirty-one clinical tissue samples were detected and compared with PRV qPCR to evaluate the diagnostic performance of the method. The diagnostic coincidence rate of the two methods was 100 %.
Taking together, we established a rapid and convenient RAA-Cas12b method for the detection of wild-type PRV. This method holds a broad application prospect in the point-of-care testing (POCT).
Links
Where it is published
- DOI doi.org/10.57760/sciencedb.16909 ↗
DOI / persistent id · from scidb cn
Catalogue records · 1
- OAI-PMH record scidb.cn/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=10.57760%2… ↗
metadata API · from scidb cn
Topics
- From keywords
- Earth & Environmental Science · Engineering · Humanities · Life Sciences · Social Science
- Inferred from text
- Disease 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ScienceDB | 10.57760/sciencedb.16909 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · scidb cn | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:earth-environmental | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:engineering | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:humanities | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| concepts[field].local:field:social-science | mapping · scidb cn | connector:scidb_cn@1.0.0 | |
| description | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/description |
| license_text | source · scidb cn | connector:scidb_cn@1.0.0 | |
| publication_date | source · scidb cn | connector:scidb_cn@1.0.0 | |
| title | source · scidb cn | connector:scidb_cn@1.0.0 | /metadata/dc/title |