Integrative serum multi-omics identifies exposure-mediated lipid dysregulation as a unifying molecular signature of male infertility

Fasting serum from 231 men (112 male infertility, 119 normozoospermic fertile controls, classified per WHO 2021) was profiled by three LC–MS workflows (HILIC/reversed-phase metabolomics, lipidomics, and a targeted 80-chemical exposomics panel), each acquired in positive and negative ionization modes.

After excluding non-shared samples, 251 complete-case samples (24 QC + 112 MI + 115 NC) were retained across all three omic layers. The package contains 1 004 mzXML files (four acquisition folders), six processed feature tables (249 metabolomic features, 2 687 lipid species across 32 classes, and 80 exposomic features), two LC–MS injection-order files, an aligned clinical metadata table, and a full original-to-renamed filename map.