Data · dataset · 2026
<p>LPS-induced alterations in intestinal transport and enteroendocrine marker expression in pSIOs.</p>
Listed in DMU Figshare and UCL Research Data Repository — shown once because both records carry DOI 10.1371/journal.pone.0359086.g005
<p>(A) Immunofluorescence of pSIOs stained with DAPI (blue) to visualize nuclei and immunolabeled for SGLT1 (green), PEPT1 (green), GLUT2 (green), TGR5 (red), and GLP-1 (green).
Description
Representative untreated (upper rows) and LPS-treated (lower rows) organoids are shown. Untreated organoids exhibited distinct localization of SGLT1, PEPT1, GLUT2, and TGR5 along the epithelial membrane with strong expression and cytoplasmic GLP-1 expression in scattered enteroendocrine-like cells within the epithelial layer.
LPS-treated organoids exhibited reduced expression intensity, irregular protein distribution, and partial loss of membrane localization. Enlarged views of the yellow boxed regions further highlight differences in expression patterns and subcellular localization between untreated and LPS-treated organoids. Images are representative of three independent biological replicates (n = 3), with at least five organoids analyzed for each treatment condition in each biological replicate.
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Scale bars: 50 μm. (B) qRT-PCR analysis showing significant alterations in nutrient transporter and enteroendocrine-related gene expression following LPS treatment. Decreased <i>SLC5A1</i> (SGLT1) and <i>SLC15A1</i> (PEPT1) expression and increased <i>SLC2A2</i> expression were observed after LPS exposure.
Among enteroendocrine-related genes, GPBAR1 expression was markedly decreased, whereas GCG (the precursor gene of GLP-1) expression was significantly increased after LPS exposure.Data are presented as the mean ± SD from three biological replicates (n = 3); each biological replicate was analyzed using five technical replicates. **<i>p</i> < 0.01, ***<i>p</i> < 0.001.</p>
Links
Where it is published
- DOI doi.org/10.1371/journal.pone.0359086.g005 ↗
DOI / persistent id · from figshare dmu ac uk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare dmu ac uk
Topics
- From keywords
- Biochemistry and cell biology · Biochemistry and cell biology · Chemistry · Chemistry · Earth & Environmental Science · Earth & Environmental Science · Genetics · Genetics · Immunology · Immunology · Infectious diseases · Infectious diseases · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health · Microbiology · Microbiology · Research, science and technology policy · Research, science and technology policy
- Inferred from text
- Image 65%
Provenance · 2 source records, 26 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| DMU Figshare | oai:figshare.com:article/34048156 | 10 d ago | JSON v1 |
| UCL Research Data Repository | oai:figshare.com:article/34048156 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].anzsrc:field:320211 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:320211 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Infectious Diseases'] |
| concepts[field].anzsrc:field:440710 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:field:440710 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Science Policy'] |
| concepts[field].anzsrc:group:3101 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3101 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cell Biology'] |
| concepts[field].anzsrc:group:3105 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3105 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Genetics'] |
| concepts[field].anzsrc:group:3107 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].anzsrc:group:3204 | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · rdr ucl ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:chemistry | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · rdr ucl ac uk | connector:rdr_ucl_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[modality].local:modality:image | enrichment · figshare dmu ac uk | keyword-concept-rules@1.0.0 | title+description (65%) |
| description | source · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | /metadata/dc/description |
| license | source · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| title | source · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | /metadata/dc/title |