Gene expression profiling of renal tissues isolated from podocyte conditional ERRα knockin or wild-type mice injected with nephrotoxic serum

To investigate the functional role and molecular mechanisms of ERRα in glomerular injury, we generated podocyte-specific ERRα conditional knock-in (cKI) mice. Our studies revealed that ERRα expression was significantly upregulated in podocytes under both in vitro and in vivo injury conditions.

Notably, podocyte-specific overexpression of ERRα exacerbated renal damage in mice subjected to nephrotoxic serum (NTS)-induced glomerular injury. Through comprehensive RNA-sequencing analysis, we further elucidated the underlying molecular mechanisms associated with podocyte-specific ERRα overexpression.