<p>Entry of bat sarbecoviruses by cell-cell fusion.</p>

HEK293T cells stably expressing half of a split GFP-Renilla (RLuc-GFP 1−7) luciferase reporter were transfected with bat sarbecovirus Spikes, and co-cultured with BHK-21 cells expressing the other half of the split reporter (RLuc-GFP 8−11), transfected with bat ACE2s. Productive cell-cell fusion results in reconstitution of the split reporter, from which luciferase signals can be measured.

Data is shown as log (RLU) and is the average of three separate experiments. A pDISPLAY vector control was included to set the baseline value to account for background signals. The red boxes indicate the predicted cognate bat receptor for each bat sarbecovirus, where known. <b>(B)</b> XY scatter plots for each of the viruses assessed for their receptor usage profile, correlating the log (RLU) of pseudoparticle entry (X) against cell-cell fusion entry (Y), excluding clade II viruses that do not use ACE2 as their entry receptor (RfGB02: <i>r</i> = 0.8244, <i>p</i> < 0.0001; RhGB07: <i>r</i> = 0.9335, <i>p</i> < 0.0001; Rc-o319: <i>r</i> = 0.9467, <i>p</i> < 0.0001; SARS-CoV-2: <i>r</i> = 0.6684, <i>p</i> = 0.0024; BANAL-20–103: <i>r</i> = 0.5308, <i>p</i> = 0.0234; BANAL-20–236: <i>r</i> = 0.6096, <i>p</i> = 0.0072; BANAL-20–52: <i>r</i> = 0.7343, <i>p</i> = 0.0005; RaTG13: <i>r</i> = 0.7719, <i>p</i> = 0.0002; SARS-CoV-1: <i>r</i> = 0.9005, <i>p</i> < 0.0001; WIV-1: <i>r</i> = 0.7724, <i>p</i> = 0.0002; Rs4231: <i>r</i> = 0.8259, <i>p</i> < 0.0001).