Enhancement of Triticum aestivum sulfamethoxazole resistance by Chlorella sorokiniana.

To clarify the enhancing effect and related mechanisms of applying Chlorella sorokiniana on improving the resistance of wheat (Triticum aestivum) seedling under sulfamethoxazole (SMX) stress, experiments on C. sorokiniana cultivation and wheat seedling hydroponics were conducted respectively: 0, 25, 50, 100 and 200 mg·L-1 SMX were added to the culture medium of C. sorokiniana for 7 days cultivation, then algal growth, photosynthetic activity, and SMX removal capacity were analyzed; Subsequently, 0, 25, 50, 100 mg·L-1 of SMX and C. sorokiniana culture solution (0.5 g·L-1) were applied to the hydroponic solution of wheat seedlings for 15 days cultivation.

The growth, photosynthetic characteristics, and SMX content in both the seedings and culture medium were measured. The results showed that C. sorokiniana exhibited strong tolerance to SMX, maintaining effective growth under mg·L-1 SMX. After 7 days cultivation, the maximum net removal rate of SMX from the culture medium reached 64.1%, with degradation being the main removal pathway under lower SMX concentrations.