Taxus cuspidata extract attenuates allergic airway inflammation, and its constituent taxinine targets the IL-17/TNF program in mast cells

To define, transcriptome-wide, how the Taxus cuspidata-derived diterpenoid taxinine modulates mast-cell activation, we profiled bone-marrow-derived mast cells (BMMCs) from C57BL/6 mice in three conditions: untreated control, IgE-sensitized cells stimulated with DNP-BSA (BSA), and DNP-BSA-stimulated cells treated with taxinine. Differential expression, functional enrichment, and gene-set enrichment analysis showed that IgE/antigen stimulation induced a strong inflammatory signature that taxinine partially attenuated, with the IL-17 and TNF signaling programs enriched by stimulation and reciprocally suppressed by taxinine.